New fluorescence method for monitoring of GPCR

A new fluorescence method for monitoring of GPCR aggregation in intact cells is the number 1 of top 20 articles in the research domain according to BioMedLib

by Monica Langfritz-Gütermann

In 2011, the Molecular Pharmacology research team developed a new method to detect GPCR aggregation in intact cells by fluorescence resonance energy transfer, FRET.
The method relies on a cleavable signal peptide, which enhances translocation of fluorescent protein-labelled membrane proteins to the cell surface.
In 2014, the article was recognized as number 1 of top 20 articles in the research domain according to BioMedLib.

external pageThe full article can be found here.

Enlarged view: FRET-cell
Confocal FRET imaging of AT1R-B2R heterodimerization in intact cells
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